[Justin] covers the basics, from building a rig for running agarose gels to pouring the gels to ... like fluorescent DNA stain for visualizing the bands; we’re heartened to see that the potent ...
One important requirement for gel shift assays is that the gel-running conditions not disrupt protein-DNA interactions. These are called nondenaturing conditions, and they differ from the ...
Sephadex G-50 and Bio-Gel P-60 are ideal for purifying DNA larger than 80 nucleotides ... form of a gel and only needs to be equilibrated in running buffer before use. Sephadex is supplied as ...
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